Gulbarga University
Year of establishment:1980-81
Gulbarga - 585 106, Karnataka State - India
DEPARTMENT OF P.G. STUDIES AND RESEARCH IN BIOCHEMISTRY
Dr. K. Sreeramulu
Professor
Dept. of PG Studies and Research in Bio-Chemistry
Jnana Ganga, Gulbarga University, Gulbarga - 585 106
Karnataka, India
Tel No.: +91 8472-263289 (O)
Fax No.: +91 8472-263205/06(O)
Residence
Plot No.93,Mahalaxmi Nagar, Brahmapur, Gulbarga - 585 102, Karnataka, India
Tel No.: +91 8472 235035 (R)
+91 9449438890 (Cell)
Date of Birth: 15.06.1962
Academic Qualifications: M.Sc., Ph.D.

M.Sc.

Gulbarga University, Gulbarga Karnataka-India

1986

Ph.D.

Gulbarga University, Gulbarga Karnataka-India

1992

Areas of Specialization
Insect Biochemistry, Microbial Enzyme technolog Plant Biochemistry
Areas of Specialization

Insect Biochemistry, Halophilic enzymes and Plant biochemistry

Research Experience in various Institutions
i). Erasmus Mundus-Svaagata.EU Fellowship
Visited Department of Biotechnology, Universitat Polytecnica De Valencia, Sapin to work on plant molecular biology-cloning of GCN2 gene in Arabidopsis thaliana from Ist April to 3rd Aug, 2015 in collaboration with Prof. Jose Gadea Vacas.

ii) VGST Award for research Publication
Vison group of Science ad Technology (VGST), Dept of Information Technology, Biotechnology and Science and technology, Govt of Karnataka has given award for publishing best paper during 2010-11 under the Chairmanship of Bharat Ratna Prof. CNR. Rao.

iii) DAAD Fellowship: Worked as a DAAD fellow in the Institute of Biochemie, Medizinische Universitate zu Lubeck, Germany on “Isolation and characterization of respiratory chain complex III and its genes of extreme halophilic bacteria, Halobacterium salinarium” from        Oct, 1994 to Feb, 1996, under the guidance of Prof. Gunter Schafer.

vi) DBT Overseas Associateship Programme:Visited Department of Molecular and Cellular Biology, University of Guelph, Ontario, Canada-N1G 2W1 to work on “Interaction of insecticides with the mammalian P-glycoprotein” from 1st April to 8th, Sept, 2006 under the guidance of Prof. Frances J. Sharom.

v). Gold medal was awarded for securing Ist rank in M.Sc (Biochemistry) during 1994-1998
SERVICES IN GULBARGA UNIVERSITY, GULBARGA
(i)  Designation of the posts held 

: Lecturer in Biochemistry

(v)  Duration : 23rd Nov, 1987 to 20th June, 1998
(i) Designation : Reader
(ii) Duration : 21st June 1998 to 20th June, 2006
(i) Designation : Professor
(ii) Date since working : 1st June, 2006   Todate
Presently working as a Chairman, Department of Biochemistry, Gulbarg University, Gulbarga
Teaching Experience
1. Lecturer, Dept. of Biochemistry, Gulbarga University, Gulbarga Karnataka-India from 23rd Nov, 1987 to 20th June, 1998
2. Reader, Dept. of Biochemistry, Gulbarga University, Gulbarga Karnataka-India from 21st June 1998 to 15 June 2006
3. Professor, Dept. of Biochemistry, Gulbarga University, Gulbarga Karnataka-India from 16st June 2006 to till date.
Administrative Experience
1. Chairman Dept. Of Bio Chemistry Gulbarga University Gulbarga , 5th October11998 to  February 2001.
2. Chairman Dept. Of Bio Chemistry Gulbarga University Gulbarga 5th October 2009 to 5th October 2011.

Research Experience in various Institutions

Sl.No. Designation Institution/University Period
1. Erasmus Mundus Department of Biotechnology Svaagata.EU Fellow  Universitat Polytecnica De Valencia Sapin Ist April To 3rd Aug, 2015 
2. DBT Overseas Associateship Department of Molecular and Cellular Biology, University of Guelph, Canada 1st April To 15th Sept, 2006
3. DAAD fellow Institute of Biochemisry, Medical University Lubeck, Germany. June 1994 To Jan, 1996
4. Research Fellow (Short term) Department of Biochemistry, Indian Institute of Science, Bangalore April 1998 To July, 1998
5. As a participant for DBT workshop on Affinity chromatography Insitute for Reproductive Biology and Endocrinology, Indian Institute of Science, Bangalore 1-08-1992 To 7-8-1992
6. As a Participant for DBT workshop on Plant Molecular Biology Centre for Advanced Studies in Plant Molecular Biology, Osamania University, Hyderabad. 10-11-1992 To 30-11-1992
7. As a Participant for DBT workshop on Plant Molecular Biology & Chloroplast. Department of Biotechnology, Madurai Kamaraj University, Madurai.

1-5-1991 To 18-5-1991

Research Guidance

● Doctor of Philosophy

Sl. No.

Name of the candidate

Year of submission

Title of the thesis

1

Srinivas. R Feb, 2004 (awarded) Biochemical studies on enzymes responsible for pesticide resistance in Helicoverpa armigera

2

Vidyasagar. M

July, 2007 (awarded)

Biochemical Studies on Halophilic and Thermostable Protease from Halobacteria

3

Raju. S.

Sept, 2009

Biochemical studies on some defense related enzymes of chickpea.

4

Nagamani S Khandre

Oct, 2009

Understanding the regulation of the voltage dependent anionic channel (VDAC) and BCL-2 family proteins in a mitochondrial regulated apoptotic pathway in T-cell

5

Prakash. S

June, 2010

Biochemical studies of extracellular amylase and xylanase from halophilic bacteria.

6

Ravindra. R.

Awarded 2012

P-glycoprotein –ATPase from the insecticide resistant pest, Helicoverpa armigera-purification and characterization and its role in resistance.

7

Prakash. P

Awaded 2012

Extreme alkalophilic, halotolerant protease from Geobacillus thermoglucosidasius

8

Md. Akbar

Awarded 2013

Biochemical studies on Bioenergetics of Helicoverpa armigera.

9.

Hanumanth Gouda

Awarded Dec, 2013

Biochemical studies on defense mechanisms of chickpea with reference to salicylic acid

10.

Gayatri Devi S

Awarded on 25th Jan, 2014

Biochemical studies on catalases and polyphenol oxidases of chickpea infected by Fusarium oxysporum f. sp. ciceri

11

Vijayalakshmi S

Awarded on 13th Feb, 2014

Utilization of agricultural feed stock for the production of alcohol and oligosaccharides through microbial process.

12

Visweswar

Working

Interaction of cry 1a with gut of H. armigera

13

Jagdish

Working

Detection of Pesticides in horti products

14

Naresh Kumar

Working

Detection of pesticides/toxicants in forensic samples

15

Pravin Sangnalmat

Working

Analysis of pesticides in forensic samples

● Master of Philosophy

Sl. No.

Name of the candidate

Year

Title of the dissertation

1

C. Srinivas Reddy

1998

Halophilic protease from archaebacteria, Halobacterium salinarium

2

G. Shamsunder

1999

Biochemical studies on pesticide resistant protein, P- glycoprotein-ATPase from the resistant pest, Heliothis armigera

3

E. Keshamma

2003

Effect of spermine, salicylic acid and cinnamic acid on some defence enzyme of Chickpea JG 62 (Cicer arietinum L).

4

Venkatesh Yadav

2006

Biochemical studies on Xylanase production by Trichoderma harzianum

5

Srisail Sajjan

2007

Effect of salicylic acid on the induction of protease inhibitors in Chickpea (Cicer arietinum L.)

6

Gayatridevi S

2008

Biochemical studies on catalases of chickpea (Cicer arietinum L.)

7

Vijayalakshmi S

2009

Biochemical studies on Mannase of Bacillus halodurans PPKS2

Research Projects

Ongoing

S.No

 Title of the project

Name of the Funding Agency

Duration

Cost
(Rs)

7

Lignocellulosic ethanol production from husks of corn, peanut and coffee cherry using indigenous microbial strains DBT BT/PR10397/PBD/26/402/2013

6-10-2015 TO
6-10-2018

30,89,200

Completed

1.

Effect of polycations on oxidative phosphorylation of rat liver mitochondrion

UGC, New Delhi

1991-1993

25,000=00

2.

Inhibition of proteases by polycations

Gulbarga University, Gulbarga

1992-1993

10,000=00

3.

Purification and characterization of aextreme halophilic thermostable protease from Halobacteria

DST, New Delhi

2003-2006

15 lakhs

4.

Purification of esterase isozyme responsible for the hydrolysis of organophosphorous compounds from the resistant pest Helicoverpa armigera.

UGC, New Delhi

2005-2008

10 lakhs

5.

P-glycoprotein ATPase: Purification, characterization and its involment in  resistance mechanism of resistnant pest, Helicoverpa armigera

DST, New Delhi

2007-2010

32 lakhs

6.

Production, Purification and characterization of alkaline and thermostable xylanase from alkaliphilic Bacillus sp.

UGC, New Delhi No. F: 37-169/2009(SR)

1-2-2010 to 30-3-2013

11,18,000

Awards

1. Editorial Board Member of the International Journal "Research and Reports in Biochemistry" Dovepress January 2011
2. Award for Best Research Publication (ARP Award - 2011) conferred by the Govt. of Karnataka, Vision Group on Science and Technology, Department of Information Technology, Biotechnology and Science and Technology, Bangalore.
Research Publications

Sl. No.

Title of Paper

Authors

Name of Journal

Year of Publication

1.

Inhibition of sweet potato beta amylase by polycation.

V.H. Mulimani*
K. Sreeramulu

Curr. Sci

58, 24, 1989, 1349-52.

2.

Kinetics of inhibition of B. subtilis alpha amylase by a polycation

V.H. Mulimani*
K. Sreeramulu

Biochem. International

20, 4, 1990, 681-86

3.

Inhibition of mitochondrial oxidative phosphorylation and its electron transport pathway.

K. Sreeramulu
V.H. Mulimani*

Indian  J. Biochem. Biophys.

30, 1993, 49-53.

4.

Inhibition of trypsin and chymotrypsin by polycations.

V.H. Mulimani*
K. Sreeramulu

Proc. Indian Natl. Sci. Acad.

59, 5, 1993, 469-475.

5.

Studies of the electron transport chain of the eury arch -eon, Halobacterium salinarium: Indications for type II NADH dehydrogenase and complex III analog.

K. Sreeramulu
C.L. Schmidt
G. Schafer*
A. Anemuller

J. Bioener & Biomembr.

30, 5, 1998, 443-453.

6.

Purification and partial characterization of cytochrome C552 from Halobacterium salinarium.

K. Sreeramulu*

Indian  J. Biochem. Biophys.

40, 2003, 274-277

7.

Efficacy of Trichoderma  species against pigeonpea wilt caused by Fusarium udum Batler.

S.K. Jayalakshmi*
K. Sreeramulu
V. Benigi

J. Biol. Control

17, (1), 2003, 75-78

8

Effect of smut diaseas on yield and yield attributing characters in chickpea

S.K. Jayalakshmi*, DM Mahalinga
K. Sreeramulu

Plant Disease Research

2003, 18 (1), 68-69

9.

Halophilic protease from Halobacterium salinarium.

C. Srinivas Reddy, S.K. Jayalakshmi
K. Sreeramulu*

Indian J. Microbiol.

43, (4), 2004,
253-256

10.

Identification of factors responsible for insecticide resistance in Helicoverpa armigera.

R. Srinivas.
S.S. Udikeri S.K. Jayalakshmi
K. Sreeramulu*

Comp. Biochem. Physiol.

Part C, 137, 2004 , 261-269.

 


11.

Hydrolysis of organophospho -rus compounds by an esterase isozyme from the resistant pest Helicoverpa armigera.

 

R. Srinivas,S.K. Jayalakshmi
K. Sreeramulu.*

Indian J. Exp. Biol

42, 2004, 214-216.

12.

Effect of spermine, salicylic acid and cinnamic acid on catalase, peroxidase and PAL of chickpea.

E. Keshamma
S.K. Jaya lakshmi, K. Sreeramulu*

J. Plant. Biol

31,(1) 2004, 13-20

13.

Effect of insecticides and inhibitors on P-glycoprotein ATPase (M-type) of resistant pest Helicoverpa armigera.

R. Srinivas
S. Shyamsunder
S.K. Jaya lakshmi, K. Sreeramulu*

Curr. Sci

2005, 88, 9, 1449-1451. 

14.

Purification and characterization of an esterase  isozyme involved in hydrolysis organophosphorus compounds from  an insecticide resistant pest, Helicoverpa armigera (Lepidoptera: Noctuidae)

R. Srinivas
S.K. Jaya lakshmi; K. Sreeramulu*
N.E. Sherman
Jayasimha Rao

Biochem. Biophys. Acta

2006, 1760, 310-317

15.

Optimization of culture conditions for the production of haloalkaliphilic thermostable protease from an extremely halophilic archaeon Halogeometricum Sp. TSS101

M.Vidyasagar, S Prakash, and K. Sreeramulu*

Lett  Appl. Microbiol

2006, 43, 385-391.

16.

Purification and characterization of a thermostable, haloalkaliphilic extracellular serine protease from the extreme halophilic archaeon Halogeometricum borinquense strain TSS101

Malashetty Vidyasagar, S Prakash, Carol Litchfield and K. Sreeramulu*

Archaea

2006, 2, 51-57.

17

Stimulatory effects of  insecticides on partially purified P-glycoprotein-ATPase from the resistant pest Helicoverpa armigera

R. Aurade
S.K. Jayalakshmi &
K. Sreeramulu*

Biochem. Cell Biol,

2006, 84, 1045-1050

18

Optimization of culture conditions for the production of halothermophilic protease from an extremely halophilic bacterium Chromohalobacter sp. TVSP101

M.Vidyasagar, S Prakash, S. K. Jayalakshmi; K.  Sreeramulu*

World J Microbiol & Biotechnol

2007, 23,655-662

19

Interactions of insecticides with mammalian P-glycoprotein and their effects on its transport

K. Sreeramulu

Ranghua Liu
F.J. Sharom*

Biochem. Biophys. Acta,

2007, 1768, 1750-1757.

20

Induction of systemic acquired resistance in susceptible and resistant cultivars of chickpea (Cicer arietinum L) genotypes: A comparative study in response to salicylic acid and spermine

Raju. S, Jayalakshmi  S.K, Usharani. S; K. Sreeramulu.

 Physiol  Mol Biol. Plants

2007, 13 (1), 27-36

21

Comparative study on the induction of defense related enzymes in two different cultivars of chickpea (Cicer arietinum L) genotypes by salicylic acid, spermine and Fusarium oxysporum f. sp. Ciceri

Raju S, S.K. Jayalakshmi,
Sreeramulu K*

Aus J Crop Science,

2008; 2 (3); 121-140

 

22

Xylanase production using inexpensive agricultural wastes and its partial characterization from a halophilic Chromohalobacter sp. TPSV 101

S. Prakash, Y. Veerangouda, L. Kyoung, K. Sreeramulu*

World J. Microbiol.  Biotechnol

2009; 25, 195-204

23

Production and purification of halophilic  amylase from an extrmely halophilic bacterium Chromohalobacter israelensis TVSP 101

S. Prakash,
M. Vidyasagar
K. Sreeramulu*

Process Biochem.

2009; 44, 210-215

24

Trichoderma harzianum L1 is potent source for lytic enzymes and as an elicitor of defense enzymes in chickpea

S. K Jaya lakshmi. S. Raju, S. Usha Rani, V.I. Benagi, K. Sreeramulu*

Aus. J. Crop Science,

2009; 3 (1); 44-52

 

25

Purification and character-ization of  an extreme halothermophilic extracellular serine protease from Chromohalobacter israelensis strain TVSP 101

M. Vidyasagar

S. Prakash
K. Sreeramulu*

Brz.   J. Microbiol

 

2009; 40; 1-8

26

Differential elicitation of proteases and protease inhibitors in two different genotypes of chickpea (Cicer arietinum L) by salicylic acid and spermine

Raju S, S.K. Jayalakshmi, K. Sreeramulu *

J.  Plant Physiol

2009, 166, 1015-1022

27

Effect of organic solvents on extreme halophilic amylases from Chromohalobacter israelensis TVSP101

Prakash S,
K.   Sreeramulu *

Int J. Biochem. Biotechnol

2009, 5 (2), 115-124

28

Production and partial characterization of keratinase by free and immobilised cells of  Bacillus sp PPKS-1

P. Prakash, S.K. Jayalakshmi and K. Sreeramulu*

Appl Biochem. Biotechnol

2010, 160: 1909-1920

29

Purification and characterization of extreme alkaline, thermostable keratinase, and keratin disulfide reductase produced by Bacillus halodurans PPKs-2

P. Prakash,
SK Jayalakshmi
K. Sreeramulu*

Appl Microbiol.
Biotechnol

2010, 87(2)   625-633.

30

P-glycoprotein ATPase from the resistant pest, Helicoverpa armigera: Purification,
characterization and effect of various insecticides on its transport function

R.M.  Aurade
SK Jayalakshmi
K. Sreeramulu*

Biochimica et Biophysica Acta

2010, 1798,  1135–1143

31.

 Modulatory effects of natural curcuminoids on P-glycoprotein ATPase of insecticide-resistant pest Helicoverpa armigera (lepidopetera: Noctüidae)

Aurade. M.R,
S.K. Jayalaksmi, K. Sreeramulu*

J. Membr. Biol

2010, 236; 271-278.

32

Induction of defense related enzymes in susceptible and resistant cultivars of chickpea  by Trichoderma harzianum L1

S.K. Jayalakshmi,
Raju s, Usharani S, Benigi V.I, Sreeramulu K*

Aust. J. Crop Sci

2011, 5(7), 188-194

33

Modulation of P-glycoprotein ATPase of Helicoverpa armigera by cholesterol: Effects on ATPase activity, Interaction of pesticides and drugs 

Aurade. M.R,
S.K. Jayalakshmi, K. Sreeramulu

Arch. Insect Biochem. Physiol

2012, 79 (1), 47-60

34

Purification and characterization of dihydrolipomide dehydrogense from the insecticide resistant pest Helicoverpa armigera.

Md. Akbar,
SK Jayalakshmi
K. Sreeramulu*

Entmol. Res

2011, 41; 221-228

35

Salicylic acid is a modulator of catalase isozymes in chickpea plants infected with
Fusarium oxysporum f. sp. ciceri

Gayatridevi M
SK Jayalakshmi
K. Sreeramulu*

Plant Physiol. Biochem

52 (2012) 154-161

36

Interaction of plant cell signaling molecules, salicylic acid and jasmonic acid, with the mitochondria of Helicoverpa armigera

Md. Akbar,
S.K. Jayalakshmi,
H.C. Sharma,
K. Sreeramulu*

J. Bioener. Biomembr.

 (2012) 44:233–241

37

Purification  and   some   properties   of   low-molecular-weight   extreme   halophilic
Xylanase from  Chromo- halobacter  sp.  TPSV  101

B.   Prakash, M.  Vidyasagar,S.K.   Jayalakshmi, K.   Sreeramulu∗

J. Mol. Catalysis

2012, 74; 192–198

38

Production of alkaliphilic, halotolerent, thermostable cellulase
free xylanase by Bacillus halodurans PPKS-2 using agro waste:
single step purification and characterization

P. Prakash,
S. K. Jayalakshmi,  B. Prakash,
M. Rubul, K. Sreeramulu*

World. J Microbiol. Biotechnol

2012, 28:183–192

39

Inhibition of P-glycoprotein ATPase and its transport function
of Helicoverpa armigera by morin, quercetin and phloroglucinol

R.M. Aurade,
S.MD. AkbaR,
Hanumanth Goud, S K. Jayalakshmi
K. Sreeramulu*

Pest. Biochem. Physiol

2011, 101; 212–219

40

Methylparathion- and carbofuran-induced mitochondrial dysfunction and oxidative stress in Helicoverpa armigera (Noctuidae: Lepidoptera)

Md. Akbar,
S.K. Jayalakshmi,
H.C. Sharma,
K. Sreeramulu*

Pest. Biochem. Physiol

103 (2012) 31–37

41.

Effect of pyrethroids, permethrin and fenvalarate, on the oxidative stress of
Helicoverpa armigera

Akbar SMD Sharma HC Jayalakshmi SK3and Sreeramulu K*

World Journal of Science and Technology 2012,

2012, 2(1):01-05
ISSN: 2231 – 2587

42

 Inhibition of Catalase Isozymes of Helicoverpa armigera(Lepidoptera: Noctuidae) by Salicylic acid.

 Hanumanth Goud G Jayalakshmi S.K, ,Ravindra M Aurade , Sreeramulu K*

IOSR Journal of Pharmacy and Biological Sciences (IOSRJPBS)

Volume 3, Issue 2 (Sep-Oct 2012), PP 26-28

43

Purification and chracterization of a defense complex consisting of prophenol oxidase and cecropine from the polyphagous pest, Helicoverpa armigera.

 

 Goudru.HG,  Kumar S,. Jayalakshmi, SK
Ballal, CR Sharma HC and Sreeramulu K*

Entmol Res

43 (2013) 55–62

44

Salicylic acid and salicylic acid sensitive and insensitive catalases in different genotypes of chickpea against Fusarium oxysporum f. sp. ciceri

Gayatridevi S, Jayalskshmi SK, Mulimani VH;  Sreeramulu K*

Physiol. Mol. Biol Plants

19 (4), 2013, 529-536.

45

Production of Extremely Alkaliphilic, Halotolerent,
Detergent, and Thermostable Mannanase by the Free
and Immobilized Cells of Bacillus halodurans PPKS-2.
Purification and Characterization

S. Vijayalaxmi & P. Prakash & S. K. Jayalakshmi &
V. H. Mulimani & K. Sreeramulu*

Appl Biochem Biotechnol

Appl Biochem Biotechnol (2013) 171:382–395

46

Production of Bioethanol from Fermented Sugars
of Sugarcane Bagasse Produced by Lignocellulolytic
Enzymes of Exiguobacterium sp. VSG-1

S. Vijayalaxmi & K. A. Anu Appaiah &
S. K. Jayalakshmi & V. H. Mulimani & K. Sreeramulu*

Appl Biochem Biotechnol

Appl Biochem Biotechnol (2013) 171:246–260

47

Polyphenols from different agricultural residues: extra-ction, identification and their antioxidant properties

S. Vijayalaxmi,
SK Jayalakshmi
K.Sreeramulu*

J. Food Sci. Technol

DOI 10.1007/s13197-014-1295-9

48

Constitutive and induced polyphenol oxidases of chickpea infected by
Fusarium oxysporum f. sp.
ciceri: Purification and their physiological significance

Gayatridevi S, Jayalskshmi SK, Mulimani VH;  Sreeramulu K*

Physiol. Mol. Plant Pathol

2014, 86; 73-80

49

Mitochondrial P-Glycoprotein ATPase Contributes to Insecticide Resistance in the Cotton Bollworm, Helicoverpa armigera (Noctuidae: Lepidoptera)

S. Md Akbar,
Aurade. R.M,
H. C. Sharma
K. Sreeramulu*

Cell Biochem. Biophys

2014,  70:651–660

50

Fermentation of Bioethanol from Rice bran and wheat bran through hydrothermal pre-treatment and enzymatic hydrolysis by Micrococcus sp.

S. Vijayalaxmi, SK.Jayalakshmi
K. Sreeramulu*

Int. J. Technol & Mangement.
ISN:2278-7518

2014, Vol 7(2), 20-28

51

Elimination of gut microflora in Helicoverpa armigera confer resistance to Bacillus thuringiensis toxin proteins and transgenic crops`

R. Vishweswar,
H.C. Sharma,
Md. Akbar &
K. Sreeramulu*

 Appl Biochem. Biotechnol

DOI 10.1007/s12010-015-1841-6

52

 Persistence and dissipation of mancozeb residues in/on tomato in Bidar District of Karnataka State, India

Jagadish G,K, Jayalakshmi S.K and Sreeramulu K*.

 Issues in Biological Sciences and Pharmaceutical Research

 2015, 3(9),pp. 94-99,

53

 Evaluation of pesticide residue in rice, wheat and pulses of Bidar district Karnataka, India

 Jagadish G.K., Jaylakshmi S.K Sreeramulu K*

 Issues in Biological Sciences and Pharmaceutical Research

2015, 3(9),pp.100-106,

54

Tryptophan fluorescence quenching as a binding assay to monitor protein conformation changes in the membrane of intact mitochondria

S. MD. Akbar,  K. Sreeramulu & Hari C. Sharma*

J Bioenerg Biomembr

DOI 10.1007/s10863-016-9653-0

55

Effects of Salicylic acid treatments on heat tolerance, catalase and polyopheol oxidase enzyme activity in chickpea cv ICCV10

Gayatridevi S, Jayalskshmi SK, Sreeramulu K*

The Bioscan

2015 10(4): 1585-89

56

Activation of purified polyphenol oxidase and laccase of chickpea cv ICCV10 by sodium dodecyl sulfate

Gayatridevi S, Jayalskshmi SK, Sreeramulu K*

The Bioscan

2016 In press

57

Characterization of storage protein, arylophorin from the hemolymphof the pest, Helicoverpa armigera

Gayatridevi S,
S MD Akbar Jayalskshmi SK, Sreeramulu K*

The Bioscan

2016 In press

Scientific Papers Presented in Seminars/Conferences/Workshops/Training Programmes :
1. “Inhibition of trypsin and chymotrypsin by polycataion” V H Mulimani and K Sreeramulu, Abstract accepted for presentation to 20th FEBS meeting held at Budapest, Hungary during 19-24th, Aug , 1990
2. “Stabilising effect of polycation on mitochondria”. V H Mulimani and K Sreeramulu, paper presented in SBC, India was held at Rajasthan Agricultural College , Udaipur  during Dec, 27th –29th , 1990.
3. “Inhibition of papain by a polycation. K. Sreeramulu and V.H Mulimani , paper  presented in 9th Indian council of Chemists was held at Gulbarga University, Gulbarga during Dec, 21-23,1990.
4. “Inhibition of electron Transsport chain pathway in mitochondria by a polycation ”. K Sreeramulu and V.H. Mulimani, paper presented in 10th Indian Council of Chemists held at Goa, University, Goa during Sept, 28-30th ,1991.
5. “Extreme halophilic, thermostable protease from an Archaebacteria ”. K. Sreeramulu, C. Srinivasa Reddy and S.K jayalakshmi .paper presented in National Symposium on Research and Education in Agricultural Biochemistry in the next century and advance in Green vegetation and leaf protein research held at Gujarat Agricultural University , Junagadh during Nov,11-13th ,1998.
6. “Cytochrome c oxidase from an Archaebacterium, Halobacterium salinarium”. K. Sreeramulu, S.K. Jayalakshmi and Gunter Schafer and S. Anemuller
7. “Elucidation of electron transport pathway in an Archeabacterium, Halobacterium salinarium. K. Sreeramulu, S.K. Jayalakshmi , G. Schafter and S.Anemuller.
8. The above two papers were presented in the 13th Annual Congress of National Environmental Science Academy and Symposium on Environmental issues of 21st century, held at Dept. of Microbiology, Gulbarga University, Gulbarga during Dec, 12-14th, 1998.
9. “Studies on the electron transport chain of the Euryarchaeon Halobacterium Salinarium Indications for the type II NADH dehydrogenase and complex III analog” K Sreeramulu, Jayalakshmi S.K and Gunter Schafer. Presented in Microbial Biotechnology –Millennium’s New Vision 42nd Annual conference of association of Microbiologist of India held at Dept of Microbiology, Gulbarga University Gulbarga, 9-11.Nov.2001.
10. “P.glycoprotein –ATPase (M-type) from the resistant pest Helicoverpa armigera: purification and partial characterization ”.R. Srinivas , S K Jayalakshmi and K.Sreeramulu 70th SBC(I) .27-29 Dec,2001 held at Dept of Biochemistry Osmania University Hyderabad, India.
11. Purification and characterization of esterase isozyme form the resistant pest Helicoverpa armigera. Srinivas R, Sreeramulu and S K Jayalakshmi. 91st Indian Science Congress held at Punjab University, Chandhigarh, Jan 3-7 2004.
12. Haloalkalophilic protease from Extreme halophilic bacterium. V S Malashetty, Prakash S and K Sreeramulu. 73rd SBC (India), Nov 21-24, 2004 held at Dept of Biochemistry G.B. Panth University of Agriculture and Technology Panthnagar, Uttaranchal India.
13. Effect of Salicylic acid, Spermine and Cinnamic acid on some defense enzymes of chick pea (Cicer arientinium L) CVJG 62. E Keshamma, S K Jayalakshmi and K Sreeramulu Symposium on Biology, Biotechnology, Epidemiology and Management of plant diseases and IPS Annual Meeting South Zone 9-10th Dec 2004, Dept of Botany, Gulbarga University, Gulbarga.
14. Differential expression of antioxidative enzymes of chickpea in L550 and ICCV10 genotypes in response to salicylic acid and spermine. S Raju, S K Jayalakshmi and K. Sreeramulu. International symposium on Frontiers in Genetics and Biotechnology-Retrospect and Prospect held at Dept. of Genetics, Osmania University, Hyderabad. 8-10th Jan 2006.
15. Screening of catalase, peroxidase and polyphenol oxidase isozymes in different genotypes of chickpea and their response to salicylic acid. Paper presented at National symposium on Biotechnology Trends and Perspectives held at Dept. of Biotechnology, S.K. University, Anantapur, A.P., India 17-18th Feb.2006
16. Effect of insecticides on ATPase activity of a P-Glycoprotein in the insecticide resistant pest Helicoverpa armigera. Presented at 49th Annual meeting and conference of the Canadian Society of Biochemistry, Molecular and cellular biology held at White Oaks Conference Resort and Spa Niagara-on-the-Lake, Ontario, Canada. May 31-June 4, 2006.
17. Purification and properties of extreme halotolerant, alkaliphilic and thermostable α-      amylases from Chromohalobacter israelensis TVSP 101. Prakash. Sangolgi 1,         Malashetty Vidyasagar 1, Vineet Mahajan2, Shouche Yogesh2, Angela Corcelli3, K.      Sreeramulu1.*
18. Comparative study on the induction of defense related enzymes in two different            cultivars of chickpea (cicer arietinum L) genotypes by salicylic acid, spermine and      Fusarium oxysporum f sp ciceri . Raju. S 1, Jayalakshmi S.K 2, and Sreeramulu. K 1*
19. Interaction of insecticides with mammalian P-glycoprotein and their effect on its       transport function.  K. Sreeramulu 1 , Ronghua Liu 2 , Frances J. Sharom*
20.

P-glycoprotein ATPase from the resistant pest Helicoverpa armigera;   Purification,       characterization and reconstitution.” Ravindra Aurade, S.K. Jayalakshmi and K. Sreeramulu     

21. Production and optimization of halo tolerant alkaline protease by an alkaliphilic        Bacillus halodurans PPKS. Prakash. P and Sreeramulu. K*
22.

Production of two extracellular keratinolytic enzymes from Bacillus halodurans sp.PPKS-2 grown on raw chicken feather and human hairs. Prakash P, Jayalakshmi S.K, and Sreeramulu. K*. The paper was presented in 2nd National conference on Biotechnology for  rural and urban development (NCBIRD-2008), held during Jan 2008 in the Department of Biotechnology, Gulbarga University Gulbarga. (Karnataka).

23. Reconstitution and modulation of P-gpATPase activity of insecticide resistant pest Helicoverpa armigera by cholesterol. Ravindra Aurade M, Jayalakshmi S.K and K . Sreeramulu
24. roduction and partial characterization of extreme halophilic and thermostable xylanase from Chromohalobacter sp. TPSV101. Praksh Sangolgi and K. Sreeramulu
25. Purification and characterization of alkaline bleach stable two keratinases produced by   Bacillus sp PPKS-2. Prakash. P and K. Sreeramulu
Invited Lectures Delivered
1. Delivered talk on Industrail applications of proteases/keratinases in the National level conference on Biotechnology Advances” organized by Dept of Biotechnology, SSBT College of Engineering and Technology, Jalagon, Maharastra on 16th March, 2012.
2. Delivered talk on “Keratinases  and their applications”in the National Seminar on Challenges, Oppertunities and emerging Trends in Microbial Technology” organized by Dept of Microbilogy, Kakatiya University, Warangal (AP) on 26th March 2012.
3. Delivered talk on “Insecticide resistance I Helicoverpa armigera”in the National Seminar on “Recent discoveries in Protein Science” organized by Karnatka State Higher Education Council, Bengalure in Association with Center’s for bioscience and Innovation/Bioinforation, tumkur Univerity, tumkur on 31Jan, 2013.
4.

Delivered talk on “Insecticide/drug resistant proteins”in the UGC sponsored National conferance on “Emerging protein Technologys for formulation Solutions” organized by Dept of Microbiology, University college of Science, Tumkur University, Tumkur on 16th Oct, 2014.

5.

Delivered talk on Fermentation of Bioethanol from Rice bran” in the  International  conference on “Global Trends in engineering, Technology and Management” organized by SSBT College of Engineering and Technology, Jalagon, Maharastra on 11th  Jan, 2015.

6.

Delivered talk on “Biodegradation of Agro-industrial wastes by Microbial enzymes for the production of bioethanol” in the UGC & KSAT sponsored National conference on  “enzyme research I Agriculture, fodd and Industrial Biotechnology” organized by the Maharani’s Science College for Women, Bangalore-560 001 on 13th March, 2015

7. Delivered invited talk on “Insecticide Resistant mechanisms in Agriculturally important pest, Helicoverp armiger” in 103 Indian Science Congrss organized by the University of Mysore, Mysore during January 3rd to 7th, 2016.
Membership of Professional Organizations
1. Life Member of  Society of Biological Chemists (India), Bangalore.
List of Refresher Courses/Summer Institutes/Orientation Courses Attended

S.No

Resfresher Courses/Summer Institutes/Orientation courses attended

Courses/Summer Institutes/
Orientation

University organized

Duration

1.

UGC sponsored Refresher course

Refresher course in Biochemistry

Department of Chemistry, Poona University, Pune

18th to 31st Aug, 1992

2.

UGC sponsored Refresher course

Refresher course on bioscience

Gulbarga University, Gulbarga. Gulbarga

Sept.26 to Oct.16, 2000

3. DBT Overseas  Associateship     Department of Molecular and Cellular    Biology, University of Guelph, Canada 1st April To 15th Sept, 2006
4. DAAD fellow   Institute of Biochemisry, Medical University Lubeck, Germany. June 1994 To Jan, 1996
5. Research Fellow (Short term)   Department of Biochemistry, Indian Institute of Science, Bangalore April 1998 To July, 1998.
6. As a Participant for DBT workshop on Affinity chromatography   Insitute for Reproduction Biology and Endocrinology, Indian Institute of Science, Bangalore. 1-8-1992 To 7-8-1992
7. As a Participant for DBT workshop on Plant Molecular Biology   Centre for Advanced Studies in Plant Molecular Biology, Osamania University, Hyderbada. 10-11-1992 To 30-11-1992
8. As a Participant for DBT workshop on Plant Molecular Biology and Chloroplast.   Department of Biotechnology Madurai Kamaraj University, Madurai. 1-5-1991 To 18-5-1991