Gulbarga University
Year of establishment:1980-81
Gulbarga - 585 106, Karnataka State - India
DEPARTMENT OF P.G. STUDIES AND RESEARCH IN BIOCHEMISTRY
Dr. K. Sreeramulu
Professor
Dept. of PG Studies and Research in Bio-Chemistry
Jnana Ganga, Gulbarga University, Gulbarga - 585 106
Karnataka, India
Tel No.: +91 8472-263289 (O)
Fax No.: +91 8472-263205/06(O)
Residence
Plot No.93,Mahalaxmi Nagar, Brahmapur, Gulbarga - 585 102, Karnataka, India
Tel No.: +91 8472 235035 (R)
+91 9449438890 (Cell)
Date of Birth: 15.06.1962
Academic Qualifications: M.Sc., Ph.D.

M.Sc.

Gulbarga University, Gulbarga Karnataka-India

1986

Ph.D.

Gulbarga University, Gulbarga Karnataka-India

1992

Areas of Specialization

Insect Biochemistry, Halophilic enzymes and Plant biochemistry

Teaching Experience
1. Lecturer, Dept. of Biochemistry, Gulbarga University, Gulbarga Karnataka-India from 23rd Nov, 1987 to 20th June, 1998
2. Reader, Dept. of Biochemistry, Gulbarga University, Gulbarga Karnataka-India from 21st June 1998 to till date.
Administrative Experience
1. Chairman Dept. Of Bio Chemistry Gulbarga University Gulbarga , 5th October11998 to  February 2001.
2. Chairman Dept. Of Bio Chemistry Gulbarga University Gulbarga 5th October 2009 to 5th October 2011.

Research Experience in various Institutions

Sl.no. Designation Institution/University Period
1. DBT Overseas Associateship Department of Molecular and Cellular Biology, University of Guelph, Canada 1st April To 15th Sept, 2006
2. DAAD fellow Institute of Biochemisry, Medical University Lubeck, Germany. June 1994 To Jan, 1996
3. Research Fellow (Short term) Department of Biochemistry, Indian Institute of Science, Bangalore April 1998 To July, 1998
4. As a participant for DBT workshop on Affinity chromatography Insitute for Reproductive Biology and Endocrinology, Indian Institute of Science, Bangalore 1-08-1992 To 7-8-1992
5. As a Participant for DBT workshop on Plant Molecular Biology Centre for Advanced Studies in Plant Molecular Biology, Osamania University, Hyderabad. 10-11-1992 To 30-11-1992
6. As a Participant for DBT workshop on Plant Molecular Biology & Chloroplast. Department of Biotechnology, Madurai Kamaraj University, Madurai.

1-5-1991 To 18-5-1991

Research Guidance

● Doctor of Philosophy

Sl.No.

Name of the student

Title of the Thesis

   Year

  Srinivas R. Biochemical studies on enzymes responsible for pesticide resistance in Helicoverpa armigera Feb, 2004
  Vidyasagar M. Biochemical Studies on Halophilic and Thermostable Protease from Halobacteria July 2007
  Raju S. Biochemical studies on some defense related enzymes of chickpea. August 2009
  Nagamani Khandre Understanding the regulation of the voltage dependent anionic channel (VDAC) and BCL-2 family proteins in mitochondrial regulated apoptotic pathway in T-cell Spetmber 2009
  Prakash S. Biochemical studies of extracellular amylase and xylanase from halophilic bacteria. March 2010
  Ravindra R. P-glycoprotein –ATPase from the insecticide resistant pest, Helicoverpa armigera-purification and characterization and its role in resistance. August 2011
  Prakash P. Extreme alkalophilic, halotolerant protease from Geobacillus thermoglucosidasius August 2011
       
       

● Master of Philosophy

Sl.No.

Name of the student

Title of the Thesis

   Year

1. C. Srinivas Reddy Halophilic protease from archaebacteria, Halobacterium salinarium 1998
2. G. Shamsunder Biochemical studies on pesticide resistant protein, P- glycoprotein-ATPase from the resistant pest, Heliothis armigera 1999
3. E. Keshamma Effect of spermine, salicylic acid and cinnamic acid on some defence enzyme of Chickpea JG 62 (Cicer arietinum L). 2003
4. Venkatesh Yadav Biochemical studies on Xylanase production by Trichoderma harzianum 2006
5. Srisailnath Sajjan Effect of salicylic acid on the induction protease inhibitors in Chickpea (Cicer arietinum L.) 2007
6. Gayatridevi Biochemical Studies on catalases of chickpea  (Cicer arietinum L.)

2008

Research Projects

Ongoing

Sl.No. Title of the project Funding Agency Duration

Amount sanctioned

1. P-glycoprotein ATPase: Purification, Characterization and its involment in resistance mechanism of resistant pest, Helicoverpa armigera DST, New Delhi. 2007 -11 32 lakhs
2. Production, purification and charaterization of xylanase from Bacillus halodurans PPKS-2 UGC, New Delhi. 2009 - 11 10 lakhs
Completed
1. Effect of polycations on oxidative phosphorylation of rat liver mitochondrion UGC, New Delhi 1991-1993 25,000/-
2. Inhibition of proteases by polycations Gulbarga University, Gulbarga 1992-1993 10,000/-
3. Purification and characterization of aextreme halophilic thermostable protease from Halobacteria DST, New Delhi 2003-2006 15 lakhs
4.

Purification of esterase isozyme responsible for the hydrolysis of organophosphorous compounds from the resistant pest Helicoverpa armigera. UGC, New Delhi 2005-2008 10 lakhs

Awards

1. Honorary Editorial Board Member of the International Journal "Research and Reports in Biochemistry" Dovepress January 2011
2. Award for Best Research Publication (ARP Award - 2011) conferred by the Govt. of Karnataka, Vision Group on Science and Technology, Department of Information Technology, Biotechnology and Science and Technology, Bangalore.
Research Publications
Sl.No Title of Paper Authors Reference of Journal Year of Publication
1. Inhibition of sweet potato beta amylase by polycation. V.H. Mulimani*
K. Sreeramulu
Curr. Sci 58, 24, 1989, 1349-52.
2. Kinetics of inhibition of B. subtilis alpha amylase by a polycation V.H. Mulimani*
K. Sreeramulu
Biochem. International 20, 4, 1990, 681-86
3. Inhibition of mitochondrial oxidative phosphorylation and its electron transport pathway V.H. Mulimani*
K. Sreeramulu
Indian J. Biochem. Biophys. 30, 1993, 49-53.
4. Inhibition of trypsin and chymotrypsin by polycations. V.H. Mulimani*
K. Sreeramulu
Proc. Ind. Natl. Sci. Acad. 59, 5, 1993, 469-475.
5. Studies of the electron transport chain of the euryarcheon, Halobacterium salinarium: Indications for type II NADH dehydrogenase and complex III analog. K. Sreeramulu
C.L. Schmidt, G. Schafer*, A. Anemuller
J. Bioenerg. Biomembr. 30, 5, 1998, 443-453.
6. Purification and partial characterization of cytochrome C552 from Halobacterium salinarium. K. Sreeramulu* Indian J. Biochem. Biophys. 40, 2003, 274-277
7. Efficacy of Trichoderma species against pigeonpea wilt caused by Fusarium udum Batler. S.K. Jayalakshmi*, K. Sreeramulu, V. Benigi J. Biol. Control 17, (1), 2003, 75-78
8. Halophilic protease from Halobacterium salinarium. C. Srinivas Reddy, S.K. Jayalakshmi,K. Sreeramulu* Indian J. Microbiol. 43, (4), 2004,253-256
9. Identification of factors responsible for insecticide resistance in Helicoverpa armigera. R. Srinivas., S.S. Udikeri, S.K. Jayalakshmi, K. Sreeramulu* Comp. Biochem. Physiol. Part C, 137, 2004, 261-269.
10. Hydrolysis of organophosphorus compounds by an esterase isozyme from the resistant pest Helicoverpa armigera. R. Srinivas, S.K. Jayalakshmi, K. Sreeramulu.* Indian J. Exp. Biol 2, 2004, 214-216
11. Effect of spermine, salicylic acid and cinnamic acid on catalase, peroxidase and PAL of chickpea. E. Keshamma, S.K. Jayalakshmi, K. Sreeramulu* J. Plant. Biol 31,(1) 2004, 13-20
12. Effect of insecticides and inhibitors on P-glycoprotein ATPase (M-type) of resistant pest Helicoverpa armigera. R. Srinivas, S. Shyamasunder, S.K. Jayalakshmi, K. Sreeramulu* Curr. Sci 2005, 88, 9, 1449-1451
13. Purification and characterization of an esterase isozyme involved in hydrolysis organophosphorus compounds from an insecticide resistant pest, Helicoverpa R. Srinivas, S.K. Jayalakshmi, K. Sreeramulu*, N.E. Sherman, Jayasimha Rao Biochem. Biophys. Acta 2006, 1760, 310-317
14. Optimization of culture conditions for the production of haloalkaliphilic thermostable protease from an extremely halophilic archaeon Halogeometricum Sp. TSS101 M.Vidyasagar, S Prakash, and K. Sreeramulu* Lett Appl. Microbiol 2006, 43, 385-391.
15. Purification and characterization of a thermostable, haloalkaliphilic extracellular serine protease from the extreme halophilic archaeon Halogeometricum borinquense strain TSS101 Malashetty Vidyasagar, S Prakash, Carol Litchfield and K. Sreeramulu* Archaea 2006, 2, 51-57.
16. Stimulatory effects of insecticides on partially purified P-glycoprotein-ATPase from the resistant pest Helicoverpa armigera R. Aurade, S.K. Jayalakshmi &, K. Sreeramulu* Biochem. Cell Biol 2006, 84, 1045-1050
17. Optimization of culture conditions for the production of halothermophilic protease from an extremely halophilic bacterium Chromohalobacter sp. TVSP101 M.Vidyasagar, S Prakash, S. Jayalakshmi and K. Sreeramulu* World J Microbiol & Biotechnol On line published 2007, 23,655-662
18. Interactions of insecticides with mammalian P-glycoprotein and their effects on its transport K. Sreeramulu, Ranghua Liu, F.J. Sharom* Biochem. Biophys. Acta 2007, 1768, 1750-1757
19. Induction of systemic acquired resistance in susceptible and resistant cultivars of chickpea (Cicer arietinum L) genotypes: A comparative study in response to salicylic acid and spermine Raju. S, Jayalakshmi S.K, Usharani. S and Sreeramulu. K* Physiol Mol Biol. Plants 2007, 13 (1)  27-36
20. Comparative study on the induction of defense related enzymes in two different cultivars of chickpea (Cicer arietinum L) genotypes by salicylic acid, spermine and Fusarium oxysporum f. sp. Ciceri Raju S, S.K. Jayalakshmi, Sreeramulu K* Aus J Crop Science, 2008; 2 (3); 121-140
21. Xylanase production using inexpensive agricultural wastes and its partial characterization from a halophilic Chromohalobacter sp. TPSV 101 S. Prakash, Y. Veerangouda, L. Kyoung, K. Sreeramulu* World J. Microbio. Biotechnol, Communicated 2009; 25, 195-204
22. Production and purification of halophilic  amylase from an extrmely halophilic bacterium Chromohalobacter israelensis TVSP 101

S. Prakash, M. VidyasagarK. Sreeramulu*

Process Biochem.

2009; 44, 210-215
23. Trichoderma harzianum L1 is potent source for lytic enzymes and as an elicitor of defense enzymes in chickpea S. K Jaya lakshmi. S. Raju, S. Usha Rani, V.I. Benagi, K. Sreeramulu* Aus. J. Crop Science, 2009; 3 (1); 44-52
24. Purification and character-ization of  an extreme halothermophilic extracellular serine protease from Chromohalobacter israelensis strain TVSP 101 M. Vidyasagar,S. Prakash,K. Sreeramulu*

Brz.   J. Microbiol 2009; 40; 1-8
25. Differential elicitation of proteases and protease inhibitors in two different genotypes of chickpea (Cicer arietinum L) by salicylic acid and spermine Raju S, S.K. Jayalakshmi, K. Sreeramulu *

J.  Plant Physiol

2009, 166, 1015-102

26. Effect of organic solvents on extreme halophilic amylases from Chromohalobacter israelensis TVSP101

Prakash S, K.   Sreeramulu *

Int J. Biochem. Biotechnol 2009, 5 (2), 115-124
27. Production and partial characterization of keratinase by free and immobilised cells of  Bacillus sp PPKS-1 P. Prakash, S.K. Jayalakshmi and K. Sreeramulu*

Appl Biochem. Biotechnol

2010, 160: 1909-1920

28. Purification and characterization of extreme alkaline, thermostable keratinase, and keratin disulfide reductase produced by Bacillus halodurans PPKs-2

P. Prakash, SK Jayalakshmi,  K. Sreeramulu*

Appl Microbiol. Biotechnol

2010, 87(2)   625-633.
29.

P-glycoprotein ATPase from the resistant pest,Helicoverpa armigera: Purification, characterization and effect of various insecticides on its transport function

R.M.  Aurade,  SK Jayalakshmi, K. Sreeramulu*

Biochimica et Biophysica Acta 2010, 1798,  1135–1143
30.  Modulatory effects of natural curcuminoids on P-glycoprotein ATPase of insecticide-resistant pest Helicoverpa armigera (lepidopetera: Noctüidae)

Aurade. M.R, S.K. Jayalaksmi, K. Sreeramulu*

J. Membr. Biol

2010  236:271-278

31.

Induction of defense related enzymes in susceptible and resistant cultivars of chickpea  by Trichoderma harzianum L1

S.K. Jayalakshmi, Raju s, Usharani S, Benigi V.I, Sreeramulu K*

Aust. J. Crop. Sci 2011,5(7):885-894
32.

Modulation of P-glycoprotein ATPase of Helicoverpa armigera by cholesterol: Effects on ATPase activity, Interaction of pesticides and drugs

Aurade. M.R, S.K. Jayalakshmi, K. Sreeramulu

Arch Insect Biochem. Physiol 2011 In Press
33. Purification and characterization of dihydrolipomide dehydrogense from the insecticide resistant pest Helicoverpa armigera.

Md. Akbar, SK Jayalakshmi K. Sreeramulu*

Entamol. Res

2011, 41; 221-228

34. Production of alkaliphilic halotolerent, thermostable cellulose free xylanase by Bacillus halodurans PPKS-2 using agro wast. Single step purification and characterization.

Prakash P.,Jayalakshmi SK.,Prakash  B.,Rubul  M.,Sreeramulu K.*

World J Microbiol. Biotechnol (2012) 28:183–192
35. Inhibition of P-glycoprotein ATPase and its transport function of Helicoverpa armigera by morin, quercetin and phoroglucinol.

Aurade R.M,Md.Akbar,H. Gouda,SK Jayalakshmi,K. Sreeramulu*

Pestic. Biochem. Physiol DOI:10.1016/j.pestbp.2011.09.008-In press
36. Purification and some properties of low-molecular-weight extreme halophilic xylanse from Chromohalobacter sp. TPSV 101.

Prakash B
Jayalakshmi SK.

Sreeramulu K.*
J.Mol Cataly: Enzym B. 2012, 74; 192–198
37. Purification and characterization of two isoforms of catalases from the chickpea and their sensitivity to salicylic acid

Gayatridevi M
SK Jayalakshmi

K. Sreeramulu*
Plant Physiol. Biochem 2012, 52, 154-161
38.

Interaction of plant cell signaling molecules, salicylic acid and jasmonic acid, with the mitochondria of Helicoverpa armigera

Md. Akbar,
S.K. Jayalakshmi,
H.C. Sharma,

K. Sreeramulu*
J. Bioener. Biomembr. DOI: 10.1007/s10863-012-9405-8. In press. 2012
39. Methylparathion- and carbofuran-induced mitochondrial dysfunction and oxidative stress in Helicoverpa armigera (Noctuidae: Lepidoptera)

Md. Akbar,
S.K. Jayalakshmi,
H.C. Sharma,

K. Sreeramulu*
Pest. Biochem. Physiol

Accepted 2012

40. Chracterization of a defense complex consisting of prophenol oxidase and cecropine from the polyphagous pest, Helicoverpa armigera.

Hanumanth Goud
SK Jayalakshmi

K. Sreeramulu*
Arch. Insect. Biochem. Physiol

2012

revised
Scientific Papers Presented in Seminars/Conferences/Workshops/Training Programmes :
1. “Inhibition of trypsin and chymotrypsin by polycataion” V H Mulimani and K Sreeramulu, Abstract accepted for presentation to 20th FEBS meeting held at Budapest, Hungary during 19-24th, Aug , 1990
2. “Stabilising effect of polycation on mitochondria”. V H Mulimani and K Sreeramulu, paper presented in SBC, India was held at Rajasthan Agricultural College , Udaipur  during Dec, 27th –29th , 1990.
3. “Inhibition of papain by a polycation. K. Sreeramulu and V.H Mulimani , paper  presented in 9th Indian council of Chemists was held at Gulbarga University, Gulbarga during Dec, 21-23,1990.
4. “Inhibition of electron Transsport chain pathway in mitochondria by a polycation ”. K Sreeramulu and V.H. Mulimani, paper presented in 10th Indian Council of Chemists held at Goa, University, Goa during Sept, 28-30th ,1991.
5. “Extreme halophilic, thermostable protease from an Archaebacteria ”. K. Sreeramulu, C. Srinivasa Reddy and S.K jayalakshmi .paper presented in National Symposium on Research and Education in Agricultural Biochemistry in the next century and advance in Green vegetation and leaf protein research held at Gujarat Agricultural University , Junagadh during Nov,11-13th ,1998.
6. “Cytochrome c oxidase from an Archaebacterium, Halobacterium salinarium”. K. Sreeramulu, S.K. Jayalakshmi and Gunter Schafer and S. Anemuller
7. “Elucidation of electron transport pathway in an Archeabacterium, Halobacterium salinarium. K. Sreeramulu, S.K. Jayalakshmi , G. Schafter and S.Anemuller.
8. The above two papers were presented in the 13th Annual Congress of National Environmental Science Academy and Symposium on Environmental issues of 21st century, held at Dept. of Microbiology, Gulbarga University, Gulbarga during Dec, 12-14th, 1998.
9. “Studies on the electron transport chain of the Euryarchaeon Halobacterium Salinarium Indications for the type II NADH dehydrogenase and complex III analog” K Sreeramulu, Jayalakshmi S.K and Gunter Schafer. Presented in Microbial Biotechnology –Millennium’s New Vision 42nd Annual conference of association of Microbiologist of India held at Dept of Microbiology, Gulbarga University Gulbarga, 9-11.Nov.2001.
10. “P.glycoprotein –ATPase (M-type) from the resistant pest Helicoverpa armigera: purification and partial characterization ”.R. Srinivas , S K Jayalakshmi and K.Sreeramulu 70th SBC(I) .27-29 Dec,2001 held at Dept of Biochemistry Osmania University Hyderabad, India.
11. Purification and characterization of esterase isozyme form the resistant pest Helicoverpa armigera. Srinivas R, Sreeramulu and S K Jayalakshmi. 91st Indian Science Congress held at Punjab University, Chandhigarh, Jan 3-7 2004.
12. Haloalkalophilic protease from Extreme halophilic bacterium. V S Malashetty, Prakash S and K Sreeramulu. 73rd SBC (India), Nov 21-24, 2004 held at Dept of Biochemistry G.B. Panth University of Agriculture and Technology Panthnagar, Uttaranchal India.
13. Effect of Salicylic acid, Spermine and Cinnamic acid on some defense enzymes of chick pea (Cicer arientinium L) CVJG 62. E Keshamma, S K Jayalakshmi and K Sreeramulu Symposium on Biology, Biotechnology, Epidemiology and Management of plant diseases and IPS Annual Meeting South Zone 9-10th Dec 2004, Dept of Botany, Gulbarga University, Gulbarga.
14. Differential expression of antioxidative enzymes of chickpea in L550 and ICCV10 genotypes in response to salicylic acid and spermine. S Raju, S K Jayalakshmi and K. Sreeramulu. International symposium on Frontiers in Genetics and Biotechnology-Retrospect and Prospect held at Dept. of Genetics, Osmania University, Hyderabad. 8-10th Jan 2006.
15. Screening of catalase, peroxidase and polyphenol oxidase isozymes in different genotypes of chickpea and their response to salicylic acid. Paper presented at National symposium on Biotechnology Trends and Perspectives held at Dept. of Biotechnology, S.K. University, Anantapur, A.P., India 17-18th Feb.2006
16. Effect of insecticides on ATPase activity of a P-Glycoprotein in the insecticide resistant pest Helicoverpa armigera. Presented at 49th Annual meeting and conference of the Canadian Society of Biochemistry, Molecular and cellular biology held at White Oaks Conference Resort and Spa Niagara-on-the-Lake, Ontario, Canada. May 31-June 4, 2006.
17. Purification and properties of extreme halotolerant, alkaliphilic and thermostable α-      amylases from Chromohalobacter israelensis TVSP 101. Prakash. Sangolgi 1,         Malashetty Vidyasagar 1, Vineet Mahajan2, Shouche Yogesh2, Angela Corcelli3, K.      Sreeramulu1.*
18. Comparative study on the induction of defense related enzymes in two different            cultivars of chickpea (cicer arietinum L) genotypes by salicylic acid, spermine and      Fusarium oxysporum f sp ciceri . Raju. S 1, Jayalakshmi S.K 2, and Sreeramulu. K 1*
19. Interaction of insecticides with mammalian P-glycoprotein and their effect on its       transport function.  K. Sreeramulu 1 , Ronghua Liu 2 , Frances J. Sharom*
20.

P-glycoprotein ATPase from the resistant pest Helicoverpa armigera;   Purification,       characterization and reconstitution.” Ravindra Aurade, S.K. Jayalakshmi and K. Sreeramulu     

21. Production and optimization of halo tolerant alkaline protease by an alkaliphilic               Bacillus halodurans PPKS. Prakash. P and Sreeramulu. K*
22.

Production of two extracellular keratinolytic enzymes from Bacillus halodurans sp.PPKS-2 grown on raw chicken feather and human hairs. Prakash P, Jayalakshmi S.K, and Sreeramulu. K*. The paper was presented in 2nd National conference on Biotechnology for  rural and urban development (NCBIRD-2008), held during Jan 2008 in the Department of Biotechnology, Gulbarga University Gulbarga. (Karnataka).

  
23.

Reconstitution and modulation of P-gpATPase activity of insecticide resistant pest Helicoverpa armigera by cholesterol. Ravindra Aurade M, Jayalakshmi S.K and K . Sreeramulu

     
24.

Production and partial characterization of extreme halophilic and thermostable xylanase from Chromohalobacter sp. TPSV101. Praksh Sangolgi and K. Sreeramulu

     
25. Purification and characterization of alkaline bleach stable two keratinases produced by   Bacillus sp PPKS-2. Prakash. P and K. Sreeramulu
Membership of Professional Organizations
1. Life Member of  Society of Biological Chemists (India), Bangalore.
List of Refresher Courses/Summer Institutes/Orientation Courses Attended
Sl.No. Designation Period Institution/University
1. DBT Overseas  Associateship   1st April To          15th Sept, 2006 Department of Molecular and Cellular    Biology, University of Guelph, Canada
2. DAAD fellow June 1994 To Jan, 1996 Institute of Biochemisry, Medical University Lubeck, Germany.
3. Research Fellow (Short term) April 1998 To July, 1998. Department of Biochemistry, Indian Institute of Science, Bangalore
4. As a Participant for DBT workshop on Affinity chromatography 1-8-1992 To 7-8-1992 Insitute for Reproduction Biology and Endocrinology, Indian Institute of Science, Bangalore.
5. As a Participant for DBT workshop on Plant Molecular Biology 10-11-1992 To 30-11-1992 Centre for Advanced Studies in Plant Molecular Biology, Osamania University, Hyderbada.
6. As a Participant for DBT workshop on Plant Molecular Biology and Chloroplast. 1-5-1991 To 18-5-1991 Department of Biotechnology Madurai Kamaraj University, Madurai.